Simple high-performance liquid chromatographic method for the quantitation of 5-fluorouracil in human plasma
Identifieur interne : 001E60 ( Main/Exploration ); précédent : 001E59; suivant : 001E61Simple high-performance liquid chromatographic method for the quantitation of 5-fluorouracil in human plasma
Auteurs : P. Compagnon [France] ; L. Thiberville [France] ; N. Moore [France] ; C. Thuillez [France] ; C. Lacroix [France]Source :
- Journal of Chromatography B: Biomedical Sciences and Applications [ 0378-4347 ] ; 1996.
Abstract
A simple, rapid, specific and sensitive high-performance liquid chromatography method has been developed for quantitation of 5-fluorouracil (5-FU) in human plasma. The method involves deproteinization of a small sample volume of plasma (150 μl) followed by HPLC on a cation-exchange resin column, Aminex HPX-87H (300×7.8 mm I.D.), preceded by a similar guard cartridge with UV detection at 265 nm. This method allows a good separation of 5-FU with a retention time of 24 min and a detection limit at 25 ng/ml. The calibration curve was linear from 25 to 2000 ng/ml. The coefficient of variation was ≤4.4% for within-day reproducibility and ≤9.5% for day-to-day reproducibility.
Url:
DOI: 10.1016/0378-4347(95)00352-5
Affiliations:
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The method involves deproteinization of a small sample volume of plasma (150 μl) followed by HPLC on a cation-exchange resin column, Aminex HPX-87H (300×7.8 mm I.D.), preceded by a similar guard cartridge with UV detection at 265 nm. This method allows a good separation of 5-FU with a retention time of 24 min and a detection limit at 25 ng/ml. The calibration curve was linear from 25 to 2000 ng/ml. The coefficient of variation was ≤4.4% for within-day reproducibility and ≤9.5% for day-to-day reproducibility.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Haute-Normandie</li><li>Région Normandie</li></region><settlement><li>Le Havre Cédex</li><li>Rouen Cédex</li></settlement></list><tree><country name="France"><region name="Région Normandie"><name sortKey="Compagnon, P" sort="Compagnon, P" uniqKey="Compagnon P" first="P." last="Compagnon">P. 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